Rhipicephalus sanguineus from Hungarian dogs: Tick identification and detection of tick-borne pathogens

Vet Parasitol Reg Stud Reports. 2024 May:50:101007. doi: 10.1016/j.vprsr.2024.101007. Epub 2024 Mar 1.


The brown dog tick, Rhipicephalus sanguineus is a complex of tick species with an unsettled species concept. In Europe, R. sanguineus is considered mainly a Mediterranean tick with sporadic findings in central and northern Europe. R. sanguineus is known as a vector of a range of pathogens of medical and veterinary importance, most of which not yet reported as autochthonous in Hungary. A total of 1839 ticks collected by veterinarians from dogs and cats were obtained in Hungary. The study aims at precise determination of ticks identified as R. sanguineus and detection of pathogens in collected ticks. All ticks were morphologically determined and 169 individuals were identified as R. sanguineus. A subset of 15 ticks was selected for molecular analysis (16S rDNA, 12S rDNA, COI). Phylogenetic analyses invariably placed sequences of all three markers into a single haplotype identified as R. sanguineus sensu stricto. All 169 brown dog ticks were tested for the presence of A. platys, E. canis, R. conorii, B. vogeli and H. canis. None of the investigated ticks was positive for the screened pathogens, though A. phagocytophilum sequence was detected in a single tick.

Keywords: Anaplasma platys; Babesia vogeli; Ehrlichia canis; Rhipicephalus sanguineus; Rickettsia conorii; Tick-borne pathogens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Case Reports

MeSH terms

  • Anaplasma*
  • Animals
  • Cats / parasitology
  • Dog Diseases* / diagnosis
  • Dog Diseases* / parasitology
  • Dogs
  • Ehrlichia canis / genetics
  • Ehrlichia canis / isolation & purification
  • Female
  • Hungary
  • Male
  • Phylogeny*
  • RNA, Ribosomal*
  • RNA, Ribosomal, 16S / analysis
  • RNA, Ribosomal, 16S / genetics
  • Rhipicephalus sanguineus* / microbiology
  • Rickettsia conorii / genetics
  • Rickettsia conorii / isolation & purification
  • Tick Infestations* / parasitology
  • Tick Infestations* / veterinary
  • Tick-Borne Diseases / microbiology
  • Tick-Borne Diseases / parasitology
  • Tick-Borne Diseases / veterinary


  • RNA, Ribosomal, 16S
  • RNA, ribosomal, 12S
  • RNA, Ribosomal

Supplementary concepts

  • Anaplasma platys