Metabolomic profile of muscles from tilapia cultured in recirculating aquaculture systems and traditional aquaculture in ponds and protein stability during freeze-thaw cycles

Ruichang Gao; Lu Liu; Abdul Razak Monto; Kai Su; Hao Zhang; Tong Shi; Zhiyu Xiong; Gangchun Xu; Yongju Luo; Yulong Bao; Li Yuan

doi:10.1016/j.foodchem.2024.139325

Metabolomic profile of muscles from tilapia cultured in recirculating aquaculture systems and traditional aquaculture in ponds and protein stability during freeze-thaw cycles

Food Chem. 2024 Apr 12:451:139325. doi: 10.1016/j.foodchem.2024.139325. Online ahead of print.

Authors

Ruichang Gao¹, Lu Liu¹, Abdul Razak Monto¹, Kai Su¹, Hao Zhang¹, Tong Shi¹, Zhiyu Xiong¹, Gangchun Xu², Yongju Luo³, Yulong Bao⁴, Li Yuan⁵

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
² Key Laboratory of Freshwater, Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi, Jiangsu 214081, China.
³ Guangxi Institute of Aquatic Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Farming, Nanning, Guangxi 530021, China.
⁴ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: yulong@ujs.edu.cn.
⁵ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: yuanli24@163.com.

PMID: 38657519
DOI: 10.1016/j.foodchem.2024.139325

Abstract

Muscle protein stability during freeze-thaw (F-T) cycles was investigated with tilapia cultured in recirculating aquaculture systems (RAS) and traditional aquaculture in ponds (TAP). This study found that fatty acids (eg., palmitic acid) were enriched in TAP, while antioxidants (eg., glutathione) were enriched in RAS. Generally, proteins in the RAS group exhibited greater stability against denaturation during the F-T cycle, suggested by a less decrease in haem protein content (77% in RAS and 86% in TAP) and a less increase in surface hydrophobicity of sarcoplasmic protein (63% in RAS and 101% in TAP). There was no significant difference in oxidative stability of myofibrillar protein between the two groups. This study provides a theoretical guide for the quality control of tilapia cultured in RAS during frozen storage.

Keywords: Aquaculture mode; Freeze-thaw; Metabolomics; Protein denaturation; Protein oxidation.