KIAA0040 enhances glioma growth by controlling the JAK2/STAT3 signalling pathway

Jie He; Kaming Xue; Fei Fan; Lin Li; Xinyu Rao; Wei Liu; Chuansheng Nie

doi:10.1111/jcmm.18332

KIAA0040 enhances glioma growth by controlling the JAK2/STAT3 signalling pathway

J Cell Mol Med. 2024 Apr;28(8):e18332. doi: 10.1111/jcmm.18332.

Authors

Jie He¹, Kaming Xue², Fei Fan¹, Lin Li¹, Xinyu Rao¹, Wei Liu³, Chuansheng Nie¹

Affiliations

¹ Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
² Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
³ Department of Information and Data Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Abstract

The role of KIAA0040 role in glioma development is not yet understood despite its connection to nervous system diseases. In this study, KIAA0040 expression levels were evaluated using qRT-PCR, WB and IHC, and functional assays were conducted to assess its impact on glioma progression, along with animal experiments. Moreover, WB was used to examine the impact of KIAA0040 on the JAK2/STAT3 signalling pathway. Our study found that KIAA0040 was increased in glioma and linked to tumour grade and poor clinical outcomes, serving as an independent prognostic factor. Functional assays showed that KIAA0040 enhances glioma growth, migration and invasion by activating the JAK2/STAT3 pathway. Of course, KIAA0040 enhances glioma growth by preventing tumour cell death and promoting cell cycle advancement. Our findings suggest that targeting KIAA0040 could be an effective treatment for glioma due to its role in promoting aggressive tumour behaviour and poor prognosis.

Keywords: JAK2/STAT3; KIAA0040; gliomas; proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Neoplasms / genetics
Brain Neoplasms / metabolism
Brain Neoplasms / pathology
Cell Line, Tumor
Cell Movement* / genetics
Cell Proliferation* / genetics
Female
Gene Expression Regulation, Neoplastic*
Glioma* / genetics
Glioma* / metabolism
Glioma* / pathology
Humans
Janus Kinase 2* / genetics
Janus Kinase 2* / metabolism
Male
Mice
Mice, Nude
Middle Aged
Prognosis
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism
STAT3 Transcription Factor* / genetics
STAT3 Transcription Factor* / metabolism
Signal Transduction*

Substances

JAK2 protein, human
Janus Kinase 2
RNA, Long Noncoding
STAT3 protein, human
STAT3 Transcription Factor
KIAA0040 protein, human