DNA Isolation Long-Read Genomic Sequencing in Ctenophores

Methods Mol Biol. 2024:2757:185-200. doi: 10.1007/978-1-0716-3642-8_7.


Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora. Specifically, we describe a Pacific Biosciences library construction method used in conjunction with gDNA isolation methods that have proven successful in obtaining high-quality genomic assemblies in ctenophores.

Keywords: Basal metazoans; Beroe; Bolinopsis; Ctenophora; Evolution; Genome; Long-read sequencing; Mnemiopsis; PacBio sequencing; Pleurobrachia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ctenophora* / genetics
  • DNA* / genetics
  • DNA* / isolation & purification
  • Gene Library
  • Genome / genetics
  • Genomics* / methods
  • High-Throughput Nucleotide Sequencing / methods
  • Sequence Analysis, DNA* / methods


  • DNA