Recording Cilia Activity in Ctenophores

Methods Mol Biol. 2024:2757:307-313. doi: 10.1007/978-1-0716-3642-8_14.

Abstract

Pelagic ctenophores swim in the water with the help of eight rows of long fused cilia. Their entire behavioral repertoire is dependent to a large degree on coordinated cilia activity. Therefore, recording cilia beating is paramount to understanding and registering the behavioral responses and investigating its neural and hormonal control. Here, we present a simple protocol to monitor and quantify cilia activity in semi-intact ctenophore preparations (using Pleurobrachia and Bolinopsis as models), which includes a standard electrophysiological setup for intracellular recording.

Keywords: Behavior; Bolinopsis; Cilia; Ctenophora; Electrophysiology; Locomotion; Pleurobrachia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cilia* / physiology
  • Ctenophora* / physiology
  • Electrophysiological Phenomena
  • Electrophysiology / methods