In situ studies of membrane biology by cryo-electron tomography

Jenny Keller; Rubén Fernández-Busnadiego

doi:10.1016/j.ceb.2024.102363

In situ studies of membrane biology by cryo-electron tomography

Curr Opin Cell Biol. 2024 Jun:88:102363. doi: 10.1016/j.ceb.2024.102363. Epub 2024 Apr 26.

Authors

Jenny Keller¹, Rubén Fernández-Busnadiego²

Affiliations

¹ University Medical Center Göttingen, Institute for Neuropathology, Göttingen, 37077, Germany; Collaborative Research Center 1190 "Compartmental Gates and Contact Sites in Cells", University of Göttingen, Göttingen, Germany. Electronic address: jenny.keller@med.uni-goettingen.de.
² University Medical Center Göttingen, Institute for Neuropathology, Göttingen, 37077, Germany; Collaborative Research Center 1190 "Compartmental Gates and Contact Sites in Cells", University of Göttingen, Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, 37077, Germany; Faculty of Physics, University of Göttingen, Göttingen, 37077, Germany. Electronic address: ruben.fernandezbusnadiego@med.uni-goettingen.de.

PMID: 38677049
DOI: 10.1016/j.ceb.2024.102363

Abstract

Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of choice to analyze the unperturbed organization of cellular membranes. Here, we briefly discuss current cryo-ET workflows and their application to study membrane biology in situ, under basal and pathological conditions.

Publication types

Review

MeSH terms

Animals
Cell Membrane* / chemistry
Cell Membrane* / metabolism
Cryoelectron Microscopy*
Electron Microscope Tomography*
Humans