Metabolomic Profiling and DNA-Fingerprinting of Newly Recorded White-Flowered Populations of Salvia lanigera Poir. in Egypt

Chem Biodivers. 2024 Jun;21(6):e202400619. doi: 10.1002/cbdv.202400619. Epub 2024 May 22.

Abstract

Salvia lanigera Poir. is a small herbaceous perennial species with violet flowers that grows in low-altitude deserts, and sandy loam. During the collection of S. lanigera, unusual populations with white flowers were found. Therefore, the two populations (violet- and white-flowered) were subjected to comparative investigations, including DNA fingerprinting, chemical composition, and biological evaluation. The two populations showed DNA variations, with 6.66 % polymorphism in ISSR and 25 % in SCoT markers. GC/MS and UHPLC/HRMS of aqueous methanol extracts, led to the tentative identification of 43 and 50 compounds in both populations. In addition, the structures of nine compounds, including four first-time reported compounds in the species, were confirmed by NMR. Furthermore, the total extracts exhibited weak radical scavenging activity against DPPH and a lower inhibitory effect towards acetylcholinesterase. In conclusion, the obtained data suggested that the white-colored flower could be an additional important character record for the Egyptian S. lanigera.

Keywords: Anti-Alzheimer's; DPPH; Fingerprinting; Phytochemical analysis; Sage.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Biphenyl Compounds / antagonists & inhibitors
  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / isolation & purification
  • Cholinesterase Inhibitors / metabolism
  • Cholinesterase Inhibitors / pharmacology
  • Chromatography, High Pressure Liquid
  • DNA Fingerprinting*
  • Egypt
  • Flowers* / chemistry
  • Flowers* / metabolism
  • Gas Chromatography-Mass Spectrometry
  • Metabolomics*
  • Picrates / antagonists & inhibitors
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Salvia* / chemistry
  • Salvia* / metabolism

Substances

  • Plant Extracts
  • Biphenyl Compounds
  • Picrates
  • 1,1-diphenyl-2-picrylhydrazyl
  • Acetylcholinesterase
  • Cholinesterase Inhibitors