Gut microbiome composition and metabolic activity in women with diverticulitis

Nat Commun. 2024 Apr 29;15(1):3612. doi: 10.1038/s41467-024-47859-4.

Abstract

The etiopathogenesis of diverticulitis, among the most common gastrointestinal diagnoses, remains largely unknown. By leveraging stool collected within a large prospective cohort, we performed shotgun metagenomic sequencing and untargeted metabolomics profiling among 121 women diagnosed with diverticulitis requiring antibiotics or hospitalizations (cases), matched to 121 women without diverticulitis (controls) according to age and race. Overall microbial community structure and metabolomic profiles differed in diverticulitis cases compared to controls, including enrichment of pro-inflammatory Ruminococcus gnavus, 1,7-dimethyluric acid, and histidine-related metabolites, and depletion of butyrate-producing bacteria and anti-inflammatory ceramides. Through integrated multi-omic analysis, we detected covarying microbial and metabolic features, such as Bilophila wadsworthia and bile acids, specific to diverticulitis. Additionally, we observed that microbial composition modulated the protective association between a prudent fiber-rich diet and diverticulitis. Our findings offer insights into the perturbations in inflammation-related microbial and metabolic signatures associated with diverticulitis, supporting the potential of microbial-based diagnostics and therapeutic targets.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Bile Acids and Salts / metabolism
  • Bilophila / metabolism
  • Case-Control Studies
  • Clostridiales / isolation & purification
  • Clostridiales / metabolism
  • Dietary Fiber / metabolism
  • Diverticulitis* / metabolism
  • Diverticulitis* / microbiology
  • Feces* / microbiology
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Metabolome
  • Metabolomics
  • Metagenomics / methods
  • Middle Aged
  • Prospective Studies

Substances

  • Bile Acids and Salts
  • Dietary Fiber

Supplementary concepts

  • Ruminococcus gnavus