Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC).
Methods: 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the receiver operating characteristic (ROC) curve was plotted to record the area under the curve (AUC).
Results: The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. Under combined detection, AUC (95% CI) = 0.950 (0.909-0.991), standard error = 0.021, and P<0.001.
Conclusions: The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.
Uvod: Cilj je bio da se istraže nivoi ekspresije i dijagnostička vrednost neurotrofnog faktora (GDNF), karcinoembrionalnog antigena (CEA) i antigena ugljenih hidrata 199 (CA199) kod pacijenata sa kolorektalnim karcinomom (CRC).
Metode: Za malignu grupu izabrano je 50 pacijenata sa CRC u našoj bolnici od februara 2020. do februara 2021. godine, za benignu grupu je izabrano još 50 pacijenata sa benignim oboljenjima debelog creva, a 50 zdravih ljudi koji su došli u našu bolnicu na fizički pregled tokom istog period su smatrani kontrolnom grupom. Periferna venska krv natašte je uzeta od svih ispitanika ujutru i testirana potpuno automatizovanim elektrohemiluminometrom da bi se odredili nivoi GDNF, CEA i CA199. Analizirana je osetljivost i specifičnost kombinovane detekcije tri indeksa za CRC, a kriva radne karakteristike prijemnika (ROC) je ucrtana da bi se zabeležila površina ispod krive (AUC).
Rezultati: Maligna grupa je imala značajno više nivoe CEA i CA199 (P<0,001) i niži nivo GDNF (P<0,001) u poređenju sa benignom i kontrolnom grupom. Osetljivost, specifičnost, pozitivna prediktivna vrednost i negativna prediktivna vrednost kombinovane detekcije iznosile su 96,0%, 94,0%, 88,9% i 97,9%, respektivno. Pod kombinovanom detekcijom, AUC (95% CI) = 0,950 (0,909-0,991), standardna greška = 0,021 i P<0,001.
Zaključak: Kombinovana dijagnoza serumskog GDNF, CEA i CA199 je pouzdana metoda za poboljšanje dijagnostičke tačnosti CRC, a ova strategija može efikasno smanjiti stopu promašene dijagnoze i ima visoku primenu u klinici.
Keywords: CA199; CEA; GDNF; colorectal carcinoma (CRC); diagnostic value.
2024 Wang Jue, Liu Lulu, Zheng Yan, Sai Gu, published by CEON/CEES.