Drosophila TET acts with PRC1 to activate gene expression independently of its catalytic activity

Sci Adv. 2024 May 3;10(18):eadn5861. doi: 10.1126/sciadv.adn5861. Epub 2024 May 3.


Enzymes of the ten-eleven translocation (TET) family play a key role in the regulation of gene expression by oxidizing 5-methylcytosine (5mC), a prominent epigenetic mark in many species. Yet, TET proteins also have less characterized noncanonical modes of action, notably in Drosophila, whose genome is devoid of 5mC. Here, we show that Drosophila TET activates the expression of genes required for larval central nervous system (CNS) development mainly in a catalytic-independent manner. Genome-wide profiling shows that TET is recruited to enhancer and promoter regions bound by Polycomb group complex (PcG) proteins. We found that TET interacts and colocalizes on chromatin preferentially with Polycomb repressor complex 1 (PRC1) rather than PRC2. Furthermore, PRC1 but not PRC2 is required for the activation of TET target genes. Last, our results suggest that TET and PRC1 binding to activated genes is interdependent. These data highlight the importance of TET noncatalytic function and the role of PRC1 for gene activation in the Drosophila larval CNS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / metabolism
  • Chromatin / genetics
  • Chromatin / metabolism
  • Drosophila / genetics
  • Drosophila / metabolism
  • Drosophila Proteins* / genetics
  • Drosophila Proteins* / metabolism
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • Gene Expression Regulation
  • Gene Expression Regulation, Developmental
  • Larva / genetics
  • Larva / metabolism
  • Polycomb Repressive Complex 1* / genetics
  • Polycomb Repressive Complex 1* / metabolism
  • Promoter Regions, Genetic
  • Protein Binding


  • Chromatin
  • Drosophila Proteins
  • Polycomb Repressive Complex 1