Plant alkaloids are nitrogen containing secondary metabolites that have wide range of biological properties including anticancer activity. 'Lasiosiphon glaucus', or 'Gnidia glauca (Fresen.) Gilg,' known for its biological properties, requires exploration to evaluate cytotoxic and anticancer effects. The present study is aimed to evaluate L. glaucus leaf alkaloid extract (LgLAE) for antimitotic, thrombolytic, and cytotoxic properties. LgLAE demonstrated comparable antimitotic efficacy to methotrexate in Allium cepa root meristematic cells. Thrombolytic evaluation showed a maximum observed clot lysis of 41.39 ± 0.21% at 2 mg/100 µL. Cytotoxicity assay shows greater inhibition of MCF-7 (144.51 μg/mL) cancer cell proliferation than MCF-10A cells (409.86 μg/mL), indicating potential cancer-specific effects. Computational analysis revealed strong binding affinities between L. glaucus alkaloids (Ergocristine, Solasodine, Solanocapsine, Delphinine, and Harmidine) and relevant receptors. These findings highlight L. glaucus contains valuable natural compounds with pharmacological effects, particularly in antimitotic, thrombolytic, and cytotoxic effects, it essential for further investigation for cancer treatment.
Keywords: Lasiosiphon glaucus; antimitotic; cytotoxicity; gnidia glauca; molecular docking; thrombolytic.