Orthopodomyia pulcripalpis is the only European representative of the tribe Orthopodomyiini (Diptera: Culicidae). Its chromosomal complement consists of three pairs of metaphase chromosomes, as it is typical of culicine mosquitoes. O. pulcripalpis shows, differently from other culicine species, polytene chromosomes of excellent cytological quality, easily obtainable from the larval salivary glands. A photomap of the polytene chromosomes of the species is described in the present paper together with some details on chromosomal polymorphism.