A20 Promoter rs5029924 Concomitant with rs2230926 and rs5029937 May Be a Prognostic Predictor for Joint Deformity or Refractory Rheumatoid Arthritis

Lihua Zhu; Lingling Zhou; Liang Wang; Cunte Chen; Jie Qiao; Xinran Huang; Xiaoyan Su; Shaohua Chen; Bo Li; Xiuli Wu; Yangqiu Li

doi:10.2147/IJGM.S461561

A20 Promoter rs5029924 Concomitant with rs2230926 and rs5029937 May Be a Prognostic Predictor for Joint Deformity or Refractory Rheumatoid Arthritis

Int J Gen Med. 2024 Apr 29:17:1707-1712. doi: 10.2147/IJGM.S461561. eCollection 2024.

Authors

Lihua Zhu^#¹, Lingling Zhou^#^{2

3}, Liang Wang^#⁴, Cunte Chen², Jie Qiao¹, Xinran Huang¹, Xiaoyan Su¹, Shaohua Chen², Bo Li², Xiuli Wu², Yangqiu Li²

Affiliations

¹ Department of Rheumatology and Immunology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China.
² Institute of Hematology, School of Medicine, Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, People's Republic of China.
³ Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510632, People's Republic of China.
⁴ Department of Oncology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China.

^# Contributed equally.

Abstract

Background: There have been several studies regarding the susceptibility of A20 gene SNPs (rs2230926 and rs5029937) in rheumatoid arthritis (RA). However, little is known about the association between polymorphisms in the A20 promoter and RA. The aim of this study was to investigate the characteristics of A20 promoter polymorphisms and the association between these polymorphisms and clinical significance in Chinese RA patients.

Methods: PCR and sequencing were used to identify A20 gene polymorphisms in peripheral blood mononuclear cells (PBMCs) from 123 RA cases and 31 healthy individuals.

Results: Only one SNP (rs5029924) in the A20 gene promoter was identified in RA patients and healthy individuals. 6 patients who carried heterozygous rs5029924 (3918C>T) together with heterozygous rs5029937 (11,571 G>T) and rs2230926 (12,486 T>G, Phe127Cys) suffered from joints deformity or refractory RA.

Conclusion: We reported the A20 promoter polymorphism rs5029924 in RA patients for the first time. rs5029924 concomitant with rs2230926 and rs5029937 may be a prognostic predictor for joint deformity or refractory RA.

Keywords: A20; RA; deformity; polymorphism; predictor; promoter; refractory.

Grants and funding

This study was supported by grants from the National Natural Science Foundation of China (Nos. 82270140), the Natural Science Foundation of Guangdong province (Nos.2017A030310456), the Funding by Science and Technology Projects in Guangzhou (Nos.2024A03J0819, 2024A03J0824), the Medical Scientific Research Foundation of Guangdong Province (Nos. A2018271 and A2019221), and the Science Research and Cultivate Foundation from the First Clinical Medical College of Jinan University (Nos. 2016320, 2017105, and 2018108).