Bacteroides vicugnae sp. nov. isolated from the fecal material of an alpaca

Samuel Miller; Meredith Hendry; Jacobey King; Krithivasan Sankaranarayanan; Paul A Lawson

doi:10.1016/j.anaerobe.2024.102862

Bacteroides vicugnae sp. nov. isolated from the fecal material of an alpaca

Anaerobe. 2024 May 7:88:102862. doi: 10.1016/j.anaerobe.2024.102862. Online ahead of print.

Authors

Samuel Miller¹, Meredith Hendry², Jacobey King³, Krithivasan Sankaranarayanan⁴, Paul A Lawson⁵

Affiliations

¹ School of Biological Sciences, 730 and 770 Van Vleet Oval, Norman, OK, 73019, USA; Laboratories of Molecular Anthropology and Microbiome Research, Stephenson Research and Technology Center, 101 David L. Boren Blvd, Norman, OK, 73019, USA. Electronic address: sammiller@ou.edu.
² School of Biological Sciences, 730 and 770 Van Vleet Oval, Norman, OK, 73019, USA. Electronic address: meredithendry@gmail.com.
³ School of Biological Sciences, 730 and 770 Van Vleet Oval, Norman, OK, 73019, USA. Electronic address: cobey@ou.edu.
⁴ School of Biological Sciences, 730 and 770 Van Vleet Oval, Norman, OK, 73019, USA; Laboratories of Molecular Anthropology and Microbiome Research, Stephenson Research and Technology Center, 101 David L. Boren Blvd, Norman, OK, 73019, USA. Electronic address: krithivasan@gmail.com.
⁵ School of Biological Sciences, 730 and 770 Van Vleet Oval, Norman, OK, 73019, USA. Electronic address: paul.lawson@ou.edu.

PMID: 38718919
DOI: 10.1016/j.anaerobe.2024.102862

Abstract

Two strictly anaerobic, Gram-stain-negative rod-shaped bacterial isolates, A2-P53^T and A1-P5, were isolated from an enrichment of fecal material from two alpacas (Vicugna pacos). Based on a comparative 16S rRNA gene sequence analysis, the isolates were assigned to the genus Bacteroides with the highest sequence similarities to Bacteroides koreensis YS-aM39T (A2- P53T 97.7 % and A1-P5 97.9 %). Additionally, the average nucleotide identity and digital DNA-DNA hybridization values between these isolates and their closest relatives within Bacteroides were less than 92.1 % and 49.1 %, respectively. The average nucleotide identity between isolates A2-P53^T and A1-P5 was 99.9 %. The predominant cellular fatty acid for isolates A2-P53^T and A1-P5 was C_15:0 antesio. The G+C % content of the isolates was 41.7 %. Based on biochemical, phylogenetic, genotypic, and chemotaxonomic criteria, these isolates A2-P53^T and A1-P5 represent two individual strains of a novel species within the genus Bacteroides for which the name Bacteroides vicugnae sp. nov. is proposed. The type strain of this species is strain A2-P53^T (CCUG 77273^T = CCM 9377^T = NRRL B-65693^T).

Keywords: 16S rRNA gene; Alpaca; Bacteroides vicugnae; Feces; Genome; Taxonomy.