Human B cell proliferation is stimulated by interleukin 2

Immunol Lett. 1985;9(2-3):105-8. doi: 10.1016/0165-2478(85)90019-7.

Abstract

The proliferation of human B cells was studied for response to interleukin 2 (IL-2) produced in Escherichia coli using recombinant DNA technology. The IL-2 was found to be an homogenous preparation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using the anti-IL-2 monoclonal antibody DMS-1. IL-2 was found to stimulate B cell proliferation. Activation of the B cells using anti-IgM antibodies increased this response. Resting T cells from the same donors were found to be less reactive to IL-2. The results suggest that human B cell proliferation can be stimulated by IL-2 alone.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Anti-Idiotypic / immunology
  • B-Lymphocytes / immunology*
  • Humans
  • Immunoglobulin M / immunology
  • In Vitro Techniques
  • Interleukin-2 / immunology*
  • Lymphocyte Activation*

Substances

  • Antibodies, Anti-Idiotypic
  • Immunoglobulin M
  • Interleukin-2