Liquid nitrogen storage of cultured T lymphocytes

P L Mottram; J L McKimm-Breschkin; J F Miller

doi:10.1016/0022-1759(85)90077-8

Liquid nitrogen storage of cultured T lymphocytes

J Immunol Methods. 1985 Apr 22;78(2):207-10. doi: 10.1016/0022-1759(85)90077-8.

Authors

P L Mottram, J L McKimm-Breschkin, J F Miller

PMID: 3872914
DOI: 10.1016/0022-1759(85)90077-8

Abstract

The conditions required for storing and recovering IL-2-dependent T cell clones from liquid nitrogen were investigated. For maximum cell recovery, cultured T lymphocytes were precooled at 4 degrees C for 15 min in medium containing 10% DMSO and 20% FCS before storage in liquid nitrogen. This method allowed adequate time for DMSO to penetrate the cells before freezing.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Cell Survival
Freezing
Humans
Preservation, Biological
T-Lymphocytes*

Grants and funding

1-RIO-A1 18439/PHS HHS/United States