Transformation of Mononuclear Phagocytes in Vivo and Malignant Histiocytosis Caused by a Novel Murine Spleen Focus-Forming Virus

Nature. 1985 May 9-15;315(6015):149-51. doi: 10.1038/315149a0.


The study of retrovirus-induced leukaemias in mice is a powerful tool for the elucidation of the normal regulation of the haematopoietic system. The acute murine spleen focus-forming viruses (SFFV) can be classified according to the haematopoietic lineage on which they exert their effects in the adult mouse. Here we report a new SFFV isolate, the AF-1 virus, with the novel ability to transform cells of the mononuclear phagocyte lineage. The virus was isolated from sarcomas that were induced on passage of a cloned Friend helper virus (F-MuLV, 643/22F) in newborn BALB/c mice. We have cloned the transforming defective subunit of the AF-1 viral complex in NRK cells and isolated several subclones. Analysis of the proviral genome in two non-producer cell clones reveals that AF-1 virus contains Harvey v-ras-specific sequences (Fig. 1). Thus, AF-1 virus is closely related to Harvey murine sarcoma virus (Ha-MSV), and is, at present, the only tool by which permanent cell lines can be obtained from mononuclear phagocytes in the mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Transformation, Viral*
  • Hematopoietic Stem Cells / microbiology
  • Histiocytosis, Langerhans-Cell / genetics
  • Histiocytosis, Langerhans-Cell / microbiology*
  • Macrophages / microbiology
  • Mice
  • Monocytes / microbiology*
  • Oncogenes*
  • Phagocytes / microbiology*
  • Retroviridae / genetics
  • Retroviridae / pathogenicity*
  • Spleen / microbiology