Anti-Photodamage Effect of Agaricus blazei Murill Polysaccharide on UVB-Damaged HaCaT Cells

Int J Mol Sci. 2024 Apr 25;25(9):4676. doi: 10.3390/ijms25094676.

Abstract

UVB radiation is known to induce photodamage to the skin, disrupt the skin barrier, elicit cutaneous inflammation, and accelerate the aging process. Agaricus blazei Murill (ABM) is an edible medicinal and nutritional fungus. One of its constituents, Agaricus blazei Murill polysaccharide (ABP), has been reported to exhibit antioxidant, anti-inflammatory, anti-tumor, and immunomodulatory effects, which suggests potential effects that protect against photodamage. In this study, a UVB-induced photodamage HaCaT model was established to investigate the potential reparative effects of ABP and its two constituents (A1 and A2). Firstly, two purified polysaccharides, A1 and A2, were obtained by DEAE-52 cellulose column chromatography, and their physical properties and chemical structures were studied. A1 and A2 exhibited a network-like microstructure, with molecular weights of 1.5 × 104 Da and 6.5 × 104 Da, respectively. The effects of A1 and A2 on cell proliferation, the mitochondrial membrane potential, and inflammatory factors were also explored. The results show that A1 and A2 significantly promoted cell proliferation, enhanced the mitochondrial membrane potential, suppressed the expression of inflammatory factors interleukin-1β (IL-1β), interleukin-8 (IL-8), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α), and increased the relative content of filaggrin (FLG) and aquaporin-3 (AQP3). The down-regulated JAK-STAT signaling pathway was found to play a role in the response to photodamage. These findings underscore the potential of ABP to ameliorate UVB-induced skin damage.

Keywords: Agaricus blazei Murill polysaccharide (ABP); UVB-damaged model; anti-photodamage; antioxidation; inflammation; structural characterization.

MeSH terms

  • Agaricus* / chemistry
  • Cell Proliferation* / drug effects
  • Cytokines / metabolism
  • Filaggrin Proteins*
  • Fungal Polysaccharides / chemistry
  • Fungal Polysaccharides / pharmacology
  • HaCaT Cells*
  • Humans
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects
  • Membrane Potential, Mitochondrial / drug effects
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology
  • Ultraviolet Rays* / adverse effects

Substances

  • Filaggrin Proteins
  • FLG protein, human
  • Fungal Polysaccharides
  • Polysaccharides
  • Cytokines

Grants and funding

This research received no external funding.