Amyloid Beta Leads to Decreased Acetylcholine Levels and Non-Small Cell Lung Cancer Cell Survival via a Mechanism That Involves p38 Mitogen-Activated Protein Kinase and Protein Kinase C in a p53-Dependent and -Independent Manner

Int J Mol Sci. 2024 May 5;25(9):5033. doi: 10.3390/ijms25095033.

Abstract

Several studies have shown an inverse correlation between the likelihood of developing a neurodegenerative disorder and cancer. We previously reported that the levels of amyloid beta (Aβ), at the center of Alzheimer's disease pathophysiology, are regulated by acetylcholinesterase (AChE) in non-small cell lung cancer (NSCLC). Here, we examined the effect of Aβ or its fragments on the levels of ACh in A549 (p53 wild-type) and H1299 (p53-null) NSCLC cell media. ACh levels were reduced by cell treatment with Aβ 1-42, Aβ 1-40, Aβ 1-28, and Aβ 25-35. AChE and p53 activities increased upon A549 cell treatment with Aβ, while knockdown of p53 in A549 cells increased ACh levels, decreased AChE activity, and diminished the Aβ effects. Aβ increased the ratio of phospho/total p38 MAPK and decreased the activity of PKC. Inhibiting p38 MAPK reduced the activity of p53 in A549 cells and increased ACh levels in the media of both cell lines, while opposite effects were found upon inhibiting PKC. ACh decreased the activity of p53 in A549 cells, decreased p38 MAPK activity, increased PKC activity, and diminished the effect of Aβ on those activities. Moreover, the negative effect of Aβ on cell viability was diminished by cell co-treatment with ACh.

Keywords: PKC; acetylcholine; acetylcholinesterase; amyloid beta; cell survival; extracellular; lung cancer; p38 MAPK; p53; signaling.

MeSH terms

  • A549 Cells
  • Acetylcholine* / metabolism
  • Acetylcholine* / pharmacology
  • Acetylcholinesterase / metabolism
  • Amyloid beta-Peptides* / metabolism
  • Carcinoma, Non-Small-Cell Lung* / genetics
  • Carcinoma, Non-Small-Cell Lung* / metabolism
  • Carcinoma, Non-Small-Cell Lung* / pathology
  • Cell Line, Tumor
  • Cell Survival* / drug effects
  • Humans
  • Lung Neoplasms* / genetics
  • Lung Neoplasms* / metabolism
  • Lung Neoplasms* / pathology
  • Protein Kinase C* / metabolism
  • Tumor Suppressor Protein p53* / metabolism
  • p38 Mitogen-Activated Protein Kinases* / metabolism

Substances

  • Acetylcholine
  • Acetylcholinesterase
  • Amyloid beta-Peptides
  • p38 Mitogen-Activated Protein Kinases
  • Protein Kinase C
  • TP53 protein, human
  • Tumor Suppressor Protein p53