Demonstration and characterization of cytosol androgen receptor in rat exorbital lacrimal gland

Biochem Int. 1985 Feb;10(2):129-35.

Abstract

The presence of a macromolecule which binds androgen with a high affinity and a low capacity was demonstrated in the cytosol of the lacrimal glands of male and female rats. Evidence was found that this macromolecule was a protein by treatment with protease, trypsin or heat. A specific 8-8.5 S peak was obtained in both sexes by glycerol gradient centrifugation in low salt condition, whereas a specific 5.2 S peak was found in high salt condition. This protein could bind to DNA-cellulose after treatment of androgen-cytosol complexes by warming (25 degrees C 15 min) or exposure under high salt (0.4 M KCl). These results suggested that this protein was an androgen receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytosol / metabolism
  • Estrenes / metabolism
  • Female
  • Kinetics
  • Lacrimal Apparatus / metabolism*
  • Male
  • Metribolone
  • Rats
  • Rats, Inbred Strains
  • Receptors, Androgen / isolation & purification
  • Receptors, Androgen / metabolism*
  • Receptors, Steroid / metabolism*
  • Testosterone Congeners / metabolism

Substances

  • Estrenes
  • Receptors, Androgen
  • Receptors, Steroid
  • Testosterone Congeners
  • Metribolone