Perinatal loss of galactosylceramidase in both oligodendrocytes and microglia is crucial for the pathogenesis of Krabbe disease in mice

Mol Ther. 2024 Jul 3;32(7):2207-2222. doi: 10.1016/j.ymthe.2024.05.019. Epub 2024 May 11.

Abstract

Lysosomal galactosylceramidase (GALC) is expressed in all brain cells, including oligodendrocytes (OLs), microglia, and astrocytes, although the cell-specific function of GALC is largely unknown. Mutations in GALC cause Krabbe disease (KD), a fatal neurological lysosomal disorder that usually affects infants. To study how Galc ablation in each glial cell type contributes to Krabbe pathogenesis, we used conditional Galc-floxed mice. Here, we found that OL-specific Galc conditional knockout (CKO) in mice results in a phenotype that includes wasting, psychosine accumulation, and neuroinflammation. Microglia- or astrocyte-specific Galc deletion alone in mice did not show specific phenotypes. Interestingly, mice with CKO of Galc from both OLs and microglia have a more severe neuroinflammation with an increase in globoid cell accumulation than OL-specific CKO alone. Moreover, the enhanced phenotype occurred without additional accumulation of psychosine. Further studies revealed that Galc knockout (Galc-KO) microglia cocultured with Galc-KO OLs elicits globoid cell formation and the overexpression of osteopontin and monocyte chemoattractant protein-1, both proteins that are known to recruit immune cells and promote engulfment of debris and damaged cells. We conclude that OLs are the primary cells that initiate KD with an elevated psychosine level and microglia are required for the progression of neuroinflammation in a psychosine-independent manner.

Keywords: Krabbe disease; MCP-1; and osteopontin; galactosylceramidase; globoid cell leukodystrophy; lysosomal storage disorder; microglia; neurodegeneration; oligodendrocytes; psychosine.

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Galactosylceramidase* / genetics
  • Galactosylceramidase* / metabolism
  • Leukodystrophy, Globoid Cell* / genetics
  • Leukodystrophy, Globoid Cell* / metabolism
  • Leukodystrophy, Globoid Cell* / pathology
  • Mice
  • Mice, Knockout*
  • Microglia* / metabolism
  • Oligodendroglia* / metabolism
  • Psychosine / metabolism

Substances

  • Galactosylceramidase
  • Psychosine