Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

Feng-Lan Liu; Rashidin Abdugheni; Cong-Guo Ran; Nan Zhou; Shuang-Jiang Liu

doi:10.1099/ijsem.0.006380

Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006380.

Authors

Feng-Lan Liu¹, Rashidin Abdugheni², Cong-Guo Ran^{3

4}, Nan Zhou³, Shuang-Jiang Liu^{3

4}

Affiliations

¹ College of Life Sciences, Hebei University, Baoding, 071000, PR China.
² State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, PR China.
³ State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center at Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
⁴ University of the Chinese Academy of Sciences, Beijing, 100049, PR China.

PMID: 38739685
DOI: 10.1099/ijsem.0.006380

Abstract

An oval to rod-shaped, Gram-stain-positive, strictly anaerobic bacterium, designated LFL-14^T, was isolated from the faeces of a healthy Chinese woman. Cells of the strain were non-spore-forming, grew optimally at 37 °C (growth range 30-45 °C) and pH 7.0 (growth range 6.0-9.0) under anaerobic conditions in the liquid modified Gifu anaerobic medium (mGAM). The result of 16S rRNA gene-based analysis indicated that LFL-14^T shared an identity of 94.7 0% with Eubacterium ventriosum ATCC 27560^T, indicating LFL-14^T represented a novel taxon. The results of genome-based analysis revealed that the average nucleotide identity (ANI), the digital DNA-DNA hybridisation (dDDH) and average amino acid identity (AAI) between LFL-14^T and its phylogenetically closest neighbour, Eubacterium ventriosum ATCC 27560^T, were 77.0 %, 24.6 and 70.9 %, respectively, indicating that LFL-14^T represents a novel species of the genus Eubacterium. The genome size of LFL-14^T was 2.92 Mbp and the DNA G+C content was 33.14 mol%. We analysed the distribution of the genome of LFL-14^T in cohorts of healthy individuals, type 2 diabetes patients (T2D) and patients with non-alcoholic fatty liver disease (NAFLD). We found that its abundance was higher in the T2D cohort, but it had a low average abundance of less than 0.2 % in all three cohorts. The percentages of frequency of occurrence in the T2D, healthy and NAFLD cohorts were 48.87 %, 16.72 % and 13.10 % respectively. The major cellular fatty acids of LFL-14^T were C_{16 : 0} (34.4 %), C_{17 : 0} 2-OH (21.4 %) and C_{14 : 0} (11.7 %). Additionally, the strain contained diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE), as well as unidentified phospholipids and unidentified glycolipids. The glucose fermentation products of LFL-14^T were acetate and butyrate. In summary, On the basis of its chemotaxonomic, phenotypic, phylogenetic and phylogenomic properties, strain LFL-14^T (= CGMCC 1.18005^T = KCTC 25580^T) is identified as representing a novel species of the genus Eubacterium, for which the name Eubacterium album sp. nov. is proposed.

Keywords: Eubacterium; Eubacterium album sp. nov.; human feces.

MeSH terms

Adult
Bacterial Typing Techniques*
Base Composition*
Butyrates / metabolism
China
DNA, Bacterial* / genetics
Eubacterium* / classification
Eubacterium* / genetics
Eubacterium* / isolation & purification
Fatty Acids*
Feces* / microbiology
Female
Genome, Bacterial
Humans
Nucleic Acid Hybridization*
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Sequence Analysis, DNA*

Substances

Fatty Acids
RNA, Ribosomal, 16S
DNA, Bacterial
Butyrates