Polymeric monolithic columns based on natural wood for rapid purification of targeted protein

Yuting Ren; Peng Ye; Limei Zhang; Liyu Zhu; Huatai Zhu; Luying Wang; Jiandu Lei; Jing Liu

doi:10.1016/j.ijbiomac.2024.132310

Polymeric monolithic columns based on natural wood for rapid purification of targeted protein

Int J Biol Macromol. 2024 May 11;270(Pt 1):132310. doi: 10.1016/j.ijbiomac.2024.132310. Online ahead of print.

Authors

Yuting Ren¹, Peng Ye¹, Limei Zhang¹, Liyu Zhu¹, Huatai Zhu¹, Luying Wang², Jiandu Lei³, Jing Liu²

Affiliations

¹ Beijing Key Laboratory of Lignocellulosic Chemistry, College of Material Science and Technology, Beijing Forestry University, Beijing 100083, China.
² Beijing Key Laboratory of Lignocellulosic Chemistry, College of Material Science and Technology, Beijing Forestry University, Beijing 100083, China; MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China.
³ Beijing Key Laboratory of Lignocellulosic Chemistry, College of Material Science and Technology, Beijing Forestry University, Beijing 100083, China; MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. Electronic address: ljd2012@bjfu.edu.cn.

PMID: 38740162
DOI: 10.1016/j.ijbiomac.2024.132310

Abstract

With multiscale hierarchical structure, wood is suitable for a range of high-value applications, especially as a chromatographic matrix. Here, we have aimed to provide a weak anion-exchange polymeric monolithic column based on natural wood with high permeability and stability for effectively separating the targeted protein. The wood-polymeric monolithic column was synthesized by in situ polymerization of glycidyl methacrylate and ethylene glycol dimethacrylate in wood, and coupled with diethylaminoethyl hydrochloride. The wood-polymeric monolithic column can be integrated with fast-protein liquid chromatography for large-scale protein purification. According to the results, the wood-polymeric monolithic column showed high hydrophilicity, permeability and stability. Separation experiments verified that the wood-polymeric monolithic column could purify the targeted protein (spike protein of SARS-COV-2 and ovalbumin) from the mixed proteins by ion exchange, and the static adsorption capacity was 33.04 mg mL^-1 and the dynamic adsorption capacity was 24.51 mg mL^-1. In addition, the wood-polymerized monolithic column had good stability, and a negligible decrease in the dynamic adsorption capacity after 20 cycles. This wood-polymerized monolithic column can provide a novel, efficient, and green matrix for monolithic chromatographic columns.

Keywords: Ovalbumin; Permeability; SARS-COV-2 spike protein; Wood-polymeric monolithic column.