T lymphocytes in infectious mononucleosis. II. Response in vitro to interleukin-2 and establishment of T cell lines

Clin Exp Immunol. 1985 Apr;60(1):70-7.

Abstract

The addition of 20% interleukin-2 (IL-2) significantly reduced the percentage of T lymphocytes dying in vitro after being isolated from the peripheral blood of acute infectious mononucleosis (IM) patients. Moreover, the immediate addition of 20% IL-2 to freshly isolated blood allowed IM T cell lines to be readily established from the peripheral blood of acute IM patients. Characterization of seven of these IM T cell lines showed them to be T3+, T11+, T4-, T9- and generally T10-. Over half of the lines characterized were T8+. It will now be possible to re-evaluate IM T cell effector functions as previous assays of IM T cell functions may have been influenced by the presence of rapid and extensive T cell death in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Survival
  • Humans
  • Infectious Mononucleosis / immunology*
  • Interleukin-2 / immunology*
  • T-Lymphocytes / classification
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology
  • Time Factors

Substances

  • Interleukin-2