SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23

Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2322974121. doi: 10.1073/pnas.2322974121. Epub 2024 May 14.


SRSF1 is the founding member of the SR protein family. It is required-interchangeably with other SR proteins-for pre-mRNA splicing in vitro, and it regulates various alternative splicing events. Dysregulation of SRSF1 expression contributes to cancer and other pathologies. Here, we characterized SRSF1's interactome using proximity labeling and mass spectrometry. This approach yielded 190 proteins enriched in the SRSF1 samples, independently of the N- or C-terminal location of the biotin-labeling domain. The detected proteins reflect established functions of SRSF1 in pre-mRNA splicing and reveal additional connections to spliceosome proteins, in addition to other recently identified functions. We validated a robust interaction with the spliceosomal RNA helicase DDX23/PRP28 using bimolecular fluorescence complementation and in vitro binding assays. The interaction is mediated by the N-terminal RS-like domain of DDX23 and both RRM1 and the RS domain of SRSF1. During pre-mRNA splicing, DDX23's ATPase activity is essential for the pre-B to B spliceosome complex transition and for release of U1 snRNP from the 5' splice site. We show that the RS-like region of DDX23's N-terminal domain is important for spliceosome incorporation, while larger deletions in this domain alter subnuclear localization. We discuss how the identified interaction of DDX23 with SRSF1 and other SR proteins may be involved in the regulation of these processes.

Keywords: DDX23; SR proteins; SRSF1; pre-mRNA splicing; proximity labeling.

MeSH terms

  • DEAD-box RNA Helicases* / genetics
  • DEAD-box RNA Helicases* / metabolism
  • HeLa Cells
  • Humans
  • Protein Binding
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • RNA Splicing
  • Serine-Arginine Splicing Factors* / genetics
  • Serine-Arginine Splicing Factors* / metabolism
  • Spliceosomes* / metabolism


  • DEAD-box RNA Helicases
  • RNA Precursors
  • Serine-Arginine Splicing Factors