Building methodological consensus to ensure rigor and reproducibility in zebrafish fertility research

M E Kossack; K Bowie; L Tian; J S Plavicki

doi:10.1016/j.aquatox.2024.106930

Building methodological consensus to ensure rigor and reproducibility in zebrafish fertility research

Aquat Toxicol. 2024 Apr 26:272:106930. doi: 10.1016/j.aquatox.2024.106930. Online ahead of print.

Authors

M E Kossack¹, K Bowie¹, L Tian¹, J S Plavicki²

Affiliations

¹ Department of Pathology and Laboratory Medicine, Brown University, United States.
² Department of Pathology and Laboratory Medicine, Brown University, United States. Electronic address: jessica_plavicki@brown.edu.

PMID: 38744123
DOI: 10.1016/j.aquatox.2024.106930

Abstract

The summary included in the text: Zebrafish are an increasingly popular model for studying the genetic and environmental factors that shape male and female fertility; however, the field currently lacks a standardized approach to fertility assessment. The current lack of consensus makes comparisons across studies more challenging and is an obstacle to reproducibility in the fields of reproductive biology and toxicology. Here, we review the diversity of spawning approaches used in zebrafish reproductive toxicology research to asses fertility and provide evidence that spawning parameters can result in meaningful differences in egg production and spawning success.

Keywords: Fertility; Reproducibility; Reproduction; Spawn; Toxicology; Zebrafish.