A biosensor method based on surfactant-mediated surface droplet evaporation for the detection of Aflatoxin B1

Supan Cheng; Fangchao Yin; Zhongxing Wang; Mei Zhao; Wenhua Ji; Jin-Ming Lin; Xiao Wang; Qiongzheng Hu

doi:10.1016/j.foodchem.2024.139635

A biosensor method based on surfactant-mediated surface droplet evaporation for the detection of Aflatoxin B₁

Food Chem. 2024 May 12:453:139635. doi: 10.1016/j.foodchem.2024.139635. Online ahead of print.

Authors

Supan Cheng¹, Fangchao Yin², Zhongxing Wang³, Mei Zhao³, Wenhua Ji³, Jin-Ming Lin⁴, Xiao Wang⁵, Qiongzheng Hu⁶

Affiliations

¹ Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; Qingdao Academy of Chinese Medical Sciences, Shandong University of Traditional Chinese Medicine, Qingdao 266112, China.
² Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address: yinfc@qlu.edu.cn.
³ Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
⁴ Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
⁵ Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address: wangx@sdas.org.
⁶ Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; Qingdao Academy of Chinese Medical Sciences, Shandong University of Traditional Chinese Medicine, Qingdao 266112, China. Electronic address: huqz@qlu.edu.cn.

PMID: 38759445
DOI: 10.1016/j.foodchem.2024.139635

Abstract

Aflatoxin B₁ (AFB₁) is a common mycotoxin that is of significant global concern due to its impact on food safety. Herein, we innovatively develop a sensing platform to detect AFB₁ based on evaporation of surfactant solutions on the hydrophobic surface, resulting in dried patterns with varied sizes. The surfactant CTAB solution produces a relatively large dried pattern due to the surface wetting. However, the reduction in the dried pattern size is found when the mixture of CTAB and AFB₁ aptamer is tested, because the formation of CTAB/aptamer complex. Moreover, the dried pattern size of the mixture of CTAB, aptamer, and AFB₁ increases due to the specific binding of AFB₁ to its aptamer. Using this innovative strategy, the AFB₁ detection can be fulfilled with a detection limit of 0.77 pg/mL. As a simple, convenient, inexpensive, and label-free method, the surfactant-mediated surface droplet evaporation-based biosensor is very promising for various potential applications.

Keywords: Aflatoxin B(1); Aptamer; Biosensor; Droplet evaporation; Surfactants.