Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells

Dou Zu-Man; Zhang Yu-Long; Tang Chun-Yang; Liu Chuang; Fang Jia-Qin; Huang Qiang; Chen Chun; You Li-Jun; Tan Chin-Ping; Niu Hui; Fu Xiong

doi:10.1016/j.foodres.2024.114428

Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells

Food Res Int. 2024 Jul:187:114428. doi: 10.1016/j.foodres.2024.114428. Epub 2024 Apr 25.

Authors

Dou Zu-Man¹, Zhang Yu-Long², Tang Chun-Yang³, Liu Chuang³, Fang Jia-Qin¹, Huang Qiang⁴, Chen Chun⁵, You Li-Jun⁶, Tan Chin-Ping⁷, Niu Hui¹, Fu Xiong⁸

Affiliations

¹ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
² Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
³ Likofu Food Co Ltd, Guangzhou Restaurant Grp, Guangzhou 511445, China.
⁴ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China.
⁵ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China. Electronic address: chenc@scut.edu.cn.
⁶ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou 510640, China.
⁷ Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Technol, Serdang 43400, Selangor, Malaysia.
⁸ SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China. Electronic address: lfxfu@scut.edu.cn.

PMID: 38763678
DOI: 10.1016/j.foodres.2024.114428

Abstract

In this study, blackberry polysaccharide-selenium nanoparticles (BBP-24-3Se) were first prepared via Na₂SeO₃/Vc redox reaction, followed by coating with red blood cell membrane (RBC) to form core-shell structure polysaccharide-selenium nanoparticles (RBC@BBP-24-3Se). The particle size of BBP-24-3Se (167.1 nm) was increased to 239.8 nm (RBC@BBP-24-3Se) with an obvious core-shell structure after coating with RBC. FT-IR and XPS results indicated that the interaction between BBP-24-3 and SeNPs formed a new C-O···Se bond with valence state of Se⁰. Bioassays indicated that RBC coating markedly enhanced both the biocompatibility and bioabsorbability of RBC@BBP-24-3Se, and the absorption rate of RBC@BBP-24-3Se in HepG2 cells was 4.99 times higher than that of BBP-24-3Se at a concentration of 10 μg/mL. Compared with BBP-24-3Se, RBC@BBP-24-3Se possessed significantly heightened protective efficacy against oxidative damage and better regulation of glucose/lipid metabolism disorder induced by palmitic acid in HepG2 cells. Mechanistic studies demonstrated that RBC@BBP-24-3Se could effectively improve PI3K/AKT signaling pathway to promote glucose metabolism, inhibit the expression of lipid synthesis genes and up-regulate the expression of lipid-decomposing genes through AMPK signaling pathway to improve lipid metabolism. These results provided a theoretical basis for developing a new type of selenium supplement for the treatment of insulin resistance.

Keywords: Blackberry polysaccharides; Core-shell structure; Glucose/lipid metabolism; Nano-selenium particles.

MeSH terms

Antioxidants / pharmacology
Glucose* / metabolism
Hep G2 Cells
Humans
Lipid Metabolism* / drug effects
Nanoparticles* / chemistry
Oxidative Stress / drug effects
Particle Size
Polysaccharides* / chemistry
Polysaccharides* / pharmacology
Rubus* / chemistry
Selenium* / chemistry
Signal Transduction / drug effects