Draft genome sequence of an ubiquinone-10 producing Methylobacterium durans LRY1-08 isolated from lichen in Thailand

Sirilak Namwong; Shingo Kato; Takao Iino; Takashi Itoh; Moriya Ohkuma; Pawina Kanchanasin; Wongsakorn Phongsopitanun; Somboon Tanasupawat

doi:10.1016/j.dib.2024.110485

Draft genome sequence of an ubiquinone-10 producing Methylobacterium durans LRY1-08 isolated from lichen in Thailand

Data Brief. 2024 May 1:54:110485. doi: 10.1016/j.dib.2024.110485. eCollection 2024 Jun.

Authors

Sirilak Namwong¹, Shingo Kato², Takao Iino², Takashi Itoh², Moriya Ohkuma², Pawina Kanchanasin³, Wongsakorn Phongsopitanun³, Somboon Tanasupawat³

Affiliations

¹ Department of Food innovation and Professional chef, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand.
² Japan Collection of Microorganisms (JCM), RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
³ Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

Abstract

A ubiquitous and pink-pigmented facultatively methylotrophic bacterium, designated LRY1-08 (=JCM 33120), was isolated from a lichen in Thailand. Strain LRY1-08 and Methylobacterium durans NBRC 112876^T shared 99.92 % similarity based on the 16S rRNA gene sequence. The draft genome of LRY1-08 was 5.26 Mbp with 4,952 protein-coding sequences and an average G + C content of 70.0 mol%. Comparing strain LRY1-08 to M. durans NBRC 112876^T, the ANIb, ANIm, AAI, and digital DNA-DNA hybridization values were 96.29 %, 97.10 %, 96.7 %, and 82.29 %, respectively. Based on the phenotypic characteristics and genome analysis, it was identified as M. durans. Its genomic sequence data revealed the PHB and CoQ10 biosynthesis genes. Therefore, the results offer suggestions for further investigation into possible applications of this bacterium in biotechnology. The draft genome was deposited at DDBJ/EMBL/GenBank (DNA Databank of Japan/European Molecular Biology Laboratory/Genbank) (JAYEEX000000000).

Keywords: Lichen; Methylobacterium; Thailand.