Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes

EMBO J. 1985 Oct;4(10):2561-8. doi: 10.1002/j.1460-2075.1985.tb03971.x.

Abstract

A cDNA clone that expresses granulocyte-macrophage colony stimulating factor (GM-CSF) activity in COS-7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin A-activated mouse helper T cell clones. Based on homology with the mouse GM-CSF cDNA sequence, the mouse GM-CSF gene was isolated. The human GM-CSF gene was also isolated based on homology with the human GM-CSF cDNA sequence. The nucleotide sequences determined for the genes and their flanking regions revealed that both the mouse and human GM-CSF genes are composed of three introns and four exons. The organization of the mouse and human GM-CSF genes are highly homologous and strong sequence homology between the two genes is found both in the coding and non-coding regions. A 'TATA'-like sequence was found 20-25 bp upstream from the transcription initiation site. In the 5'-flanking region, there is a highly homologous region extending 330 bp upstream of the putative TATA box. This sequence may play a role in regulation of expression of the GM-CSF gene. These structures are compared with those of different lymphokine genes and their regulatory regions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Colony-Stimulating Factors / genetics*
  • DNA / genetics
  • Genes
  • Granulocytes
  • Humans
  • Lymphokines / genetics
  • Macrophages
  • Mice
  • Promoter Regions, Genetic
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Colony-Stimulating Factors
  • Lymphokines
  • DNA

Associated data

  • GENBANK/X03020
  • GENBANK/X03021