Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

FEBS Lett. 1985 Oct 21;191(1):63-6. doi: 10.1016/0014-5793(85)80994-7.

Abstract

DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • Corticotropin-Releasing Hormone / biosynthesis*
  • DNA / analysis*
  • Protein Precursors / analysis
  • Protein Precursors / genetics*
  • Rats

Substances

  • Amino Acids
  • Protein Precursors
  • DNA
  • Corticotropin-Releasing Hormone

Associated data

  • GENBANK/X03036