"Thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy

Histochemistry. 1985;83(5):409-11. doi: 10.1007/BF00509201.

Abstract

Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colloids
  • Gold*
  • Histocytochemistry / methods*
  • Male
  • Microscopy, Electron
  • Pancreas / enzymology
  • Rats
  • Rats, Inbred Strains
  • Staphylococcal Protein A
  • Thiocyanates*
  • alpha-Amylases / analysis

Substances

  • Colloids
  • Staphylococcal Protein A
  • Thiocyanates
  • Gold
  • alpha-Amylases
  • thiocyanate