Swelling of the brain in mice caused by pertussis vaccine--its quantitative determination and the responsible factors in the vaccine

Jpn J Med Sci Biol. 1985 Apr;38(2):53-65. doi: 10.7883/yoken1952.38.53.


Intracerebral injection of vaccine into the mouse induced swelling of the brain. The swelling reached the maximum in the intensity by day 1 and persisted for several days. A method for quantitative determination of the brain-swelling activity of the vaccine was developed. A positive regression coefficient was found only between the brain-swelling and the lymphocytosis-promoting activities. Such activity was no longer shown with the vaccine heat-treated for 30 min at 80 C, but it was restored upon addition of the lymphocytosis-promoting factor (LPF) that caused no brain swelling by itself. The activity, therefore, was ascribed to cooperation of LPF and a certain heat-stable component other than endotoxin contained by pertussis vaccine.

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Brain / anatomy & histology
  • Brain / drug effects
  • Brain / pathology*
  • Cerebral Ventricles / drug effects
  • Cerebral Ventricles / pathology*
  • Diphtheria Toxoid / toxicity*
  • Diphtheria-Tetanus-Pertussis Vaccine
  • Dose-Response Relationship, Drug
  • Drug Combinations / toxicity
  • Injections, Intraventricular
  • Male
  • Mice
  • Mice, Inbred Strains
  • Organ Size / drug effects
  • Pertussis Vaccine / administration & dosage
  • Pertussis Vaccine / toxicity*
  • Tetanus Toxoid / toxicity*


  • Diphtheria Toxoid
  • Diphtheria-Tetanus-Pertussis Vaccine
  • Drug Combinations
  • Pertussis Vaccine
  • Tetanus Toxoid