In-cell chemical construction of a photoswitchable CENP-E using a photochromic covalent inhibitor

Kazuya Matsuo; Shusuke Yamaoka; Tomonori Waku; Akio Kobori

doi:10.1039/d4ob00647j

In-cell chemical construction of a photoswitchable CENP-E using a photochromic covalent inhibitor

Org Biomol Chem. 2024 Jun 12;22(23):4651-4655. doi: 10.1039/d4ob00647j.

Authors

Kazuya Matsuo¹, Shusuke Yamaoka¹, Tomonori Waku¹, Akio Kobori¹

Affiliation

¹ Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kmatsuo@kit.ac.jp.

PMID: 38787760
DOI: 10.1039/d4ob00647j

Abstract

An arylazopyrazole-based covalent inhibitor targeting the mitotic motor protein of centromere-associated protein E (CENP-E) was developed. Using this photoswitchable inhibitor, a photoswitchable CENP-E was chemically constructed in cells, which enabled to local control of mitotic cell division with light illumination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomal Proteins, Non-Histone* / antagonists & inhibitors
Chromosomal Proteins, Non-Histone* / metabolism
HeLa Cells
Humans
Light
Mitosis / drug effects
Molecular Structure
Photochemical Processes
Pyrazoles / chemical synthesis
Pyrazoles / chemistry
Pyrazoles / pharmacology

Substances

Chromosomal Proteins, Non-Histone
centromere protein E
Pyrazoles