Toxoplasma gondii: redistribution of monoclonal antibodies on tachyzoites during host cell invasion

Exp Parasitol. 1985 Feb;59(1):24-32. doi: 10.1016/0014-4894(85)90053-0.

Abstract

Immunodetection of protein P30, a major surface antigen of Toxoplasma gondii tachyzoites, by a specific monoclonal antibody has demonstrated a homogenous distribution of this antigen on the surface of intra- and extracellular tachyzoites at all stages of their endodyogenic development. On living zoites, no redistribution of anti-P30 was obtained, contrasting with the capping obtained with antiserum to T. gondii. Upon invasion of a host cell, however, most of the coat of anti-P30 was shed from preincubated zoites at the level of the moving junction governing the entry of the parasite into the host cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Protozoan / analysis*
  • Antigens, Protozoan / immunology
  • Antigens, Surface / analysis*
  • Antigens, Surface / immunology
  • Cell Membrane / parasitology
  • Cell Membrane / ultrastructure
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Immunoenzyme Techniques
  • Membrane Proteins / analysis
  • Membrane Proteins / immunology
  • Microscopy, Electron
  • Protozoan Proteins*
  • Toxoplasma / immunology
  • Toxoplasma / physiology*
  • Toxoplasma / ultrastructure

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • Antigens, Surface
  • Membrane Proteins
  • Protozoan Proteins
  • SAG1 antigen, Toxoplasma