Effect of LncRNA LOC106505926 on myogenesis and Lipogenesis of porcine primary cells

BMC Genomics. 2024 May 30;25(1):530. doi: 10.1186/s12864-024-10422-y.

Abstract

Background: Skeletal muscle development and fat deposition have important effects on meat quality. The study of regulating skeletal muscle development and fat deposition is of great significance in improving the quality of carcass and meat. In the present study, whole transcriptome sequencing (including RNA-Seq and miRNA-Seq) was performed on the longissimus dorsi muscle (LDM) of Jinfen White pigs at 1, 90, and 180 days of age.

Results: The results showed that a total of 245 differentially expressed miRNAs were screened in any two comparisons, which may be involved in the regulation of myogenesis. Among them, compared with 1-day-old group, miR-22-5p was significantly up-regulated in 90-day-old group and 180-day-old group. Functional studies demonstrated that miR-22-5p inhibited the proliferation and differentiation of porcine skeletal muscle satellite cells (PSCs). Pearson correlation coefficient analysis showed that long non-coding RNA (lncRNA) LOC106505926 and CXXC5 gene had strong negative correlations with miR-22-5p. The LOC106505926 and CXXC5 were proven to promote the proliferation and differentiation of PSCs, as opposed to miR-22-5p. In terms of mechanism, LOC106505926 functions as a molecular sponge of miR-22-5p to modulate the expression of CXXC5, thereby inhibits the differentiation of PSCs. In addition, LOC106505926 regulates the differentiation of porcine preadipocytes through direct binding with FASN.

Conclusions: Collectively, our results highlight the multifaceted regulatory role of LOC106505926 in controlling skeletal muscle and adipose tissue development in pigs and provide new targets for improving the quality of livestock products by regulating skeletal muscle development and fat deposition.

Keywords: LOC106505926; Lipogenesis; Myogenesis; Pig; Preadipocytes; Skeletal muscle satellite cells.

MeSH terms

  • Animals
  • Cell Differentiation* / genetics
  • Cell Proliferation
  • Cells, Cultured
  • Lipogenesis* / genetics
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Muscle Development* / genetics
  • Muscle, Skeletal / growth & development
  • Muscle, Skeletal / metabolism
  • RNA, Long Noncoding* / genetics
  • Satellite Cells, Skeletal Muscle / cytology
  • Satellite Cells, Skeletal Muscle / metabolism
  • Swine

Substances

  • RNA, Long Noncoding
  • MicroRNAs