Relative efficacy of masks and respirators as source control for viral aerosol shedding from people infected with SARS-CoV-2: a controlled human exhaled breath aerosol experimental study

EBioMedicine. 2024 Jun:104:105157. doi: 10.1016/j.ebiom.2024.105157. Epub 2024 May 30.

Abstract

Background: Tight-fitting masks and respirators, in manikin studies, improved aerosol source control compared to loose-fitting masks. Whether this translates to humans is not known.

Methods: We compared efficacy of masks (cloth and surgical) and respirators (KN95 and N95) as source control for SARS-CoV-2 viral load in exhaled breath of volunteers with COVID-19 using a controlled human experimental study. Volunteers (N = 44, 43% female) provided paired unmasked and masked breath samples allowing computation of source-control factors.

Findings: All masks and respirators significantly reduced exhaled viral load, without fit tests or training. A duckbill N95 reduced exhaled viral load by 98% (95% CI: 97%-99%), and significantly outperformed a KN95 (p < 0.001) as well as cloth and surgical masks. Cloth masks outperformed a surgical mask (p = 0.027) and the tested KN95 (p = 0.014).

Interpretation: These results suggest that N95 respirators could be the standard of care in nursing homes and healthcare settings when respiratory viral infections are prevalent in the community and healthcare-associated transmission risk is elevated.

Funding: Defense Advanced Research Projects Agency, National Institute of Allergy and Infectious Diseases, Centers for Disease Control and Prevention, the Bill & Melinda Gates Foundation, and The Flu Lab.

Keywords: Exhaled breath aerosols; Respirators; SARS-CoV-2; Source control; Surgical masks.

MeSH terms

  • Adult
  • Aerosols
  • Breath Tests / methods
  • COVID-19* / prevention & control
  • COVID-19* / transmission
  • COVID-19* / virology
  • Exhalation
  • Female
  • Humans
  • Male
  • Masks*
  • Middle Aged
  • N95 Respirators* / virology
  • Respiratory Aerosols and Droplets / virology
  • SARS-CoV-2* / isolation & purification
  • Viral Load*
  • Virus Shedding

Substances

  • Aerosols