Isolation and Characterisation of a Yeast Chromosomal Replicator

Nature. 1979 Nov 1;282(5734):39-43. doi: 10.1038/282039a0.

Abstract

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.

MeSH terms

  • Base Sequence
  • Chromosomes / analysis
  • DNA Replication*
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • DNA, Recombinant
  • Recombination, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic

Substances

  • DNA, Fungal
  • DNA, Recombinant