Hepatic triglyceride lipase in diabetic dogs

Metabolism. 1985 Mar;34(3):251-4. doi: 10.1016/0026-0495(85)90008-3.


Circulating triglyceride is cleared by a combination of hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Although LPL has been extensively studied in diabetes, the effect of insulinization on H-TGL activity has not been well characterized. To determine whether H-TGL activity is altered in insulin-deficient diabetes, postheparin plasma was obtained from eight beagle dogs: three normal (nondiabetic) control dogs and five pancreatectomized diabetic dogs were studied acutely in poor diabetic control (underinsulinized), and again in short-term good control (well insulinized). Plasma glucose, measured at the start of the studies, was 88 +/- 10 mg/100 mL (mean +/- SD) in the normal control dogs, 434 +/- 31 mL in pancreatectomized dogs in poor diabetic control, and 87 +/- 16 in good diabetic control. Peak (five minutes) postheparin plasma H-TGL activity was increased in dogs in poor diabetic control (212 +/- 43 nmol FFA/min/mL) v the normal control dogs (135 +/- 21 nmol FFA/min/mL, P less than 0.02). When the dogs were in good diabetic control, the peak H-TGL (202 +/- 40 nmol FFA/min/mL) was also significantly increased compared with the level in normal dogs, while the sum of five and 45 minute postheparin H-TGL levels for the dogs in good diabetic control was less than when they were in poor diabetic control (P less than 0.01). Thus, insulin-deficient diabetes in dogs increases H-TGL, and short-term improvement of glycemic control with insulin partially corrects this increase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Glucose
  • Diabetes Mellitus, Experimental / enzymology*
  • Diabetes Mellitus, Experimental / metabolism
  • Dogs
  • Heparin / pharmacology
  • Infusions, Parenteral
  • Insulin / metabolism*
  • Lipase / blood
  • Lipase / metabolism*
  • Lipoprotein Lipase / metabolism
  • Liver / drug effects
  • Liver / enzymology*
  • Liver / metabolism
  • Male
  • Pancreatectomy


  • Blood Glucose
  • Insulin
  • Heparin
  • Lipase
  • Lipoprotein Lipase