A nano-level assay of tizanidine using the fluorogenic character of benzofurazan derivative: Application to plasma, tablets, and content homogeneity evaluation

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Nov 5:320:124596. doi: 10.1016/j.saa.2024.124596. Epub 2024 Jun 4.


People frequently administer Tizanidine (TIZ) to treat spasticity resulting from diseases like multiple sclerosis or spinal cord injuries. It also helps prevent muscle spasms. It helps to relax and release tense and stiff muscles by inhibiting specific nerve signals in the brain and spinal cord. The technique employed in this study made use of the unique ability of benzofurazan to confer fluorescent character when reacted with TIZ at specific conditions. This fluorogenic property was harnessed to evolve a remarkably sensitive, affordable, and selective method to quantify TIZ. The resulting yellow fluorescent product was observedat a wavelength beam of 532.9 nm, and an excitation wavelength beam of 474.9 nm was applied. By looking at the response across the TIZ concentration, the calibration chart's linearity was assessed in the range of 40-500 ng/mL. By computation, the approach's detection level (LOD) was determined to be 11.9 ng/mL, while the quantitation level was approximated to be 36 ng/mL. All pertinent factors impacting the strategy's efficacy were thoroughly inspected and adjusted accordingly. The proposed strategy was validated following the guidelines outlined by the ICH. The outcomes confirmed the method's capability for the accurate quantifying of TIZ in tablets, spiked plasma, and pharmaceutical assessing content uniformity.

Keywords: Content uniformity; Determination; Plasma; Spectrofluorimetric; Tizanidine.

MeSH terms

  • Benzoxazoles* / chemistry
  • Calibration
  • Clonidine* / analogs & derivatives
  • Clonidine* / analysis
  • Clonidine* / blood
  • Fluorescent Dyes / chemistry
  • Humans
  • Hydrogen-Ion Concentration
  • Limit of Detection*
  • Reproducibility of Results
  • Spectrometry, Fluorescence* / methods
  • Tablets*


  • tizanidine
  • Tablets
  • Clonidine
  • benzofurazan
  • Benzoxazoles
  • Fluorescent Dyes