Expression in Escherichia coli of a fusion protein product containing a region of the adenovirus DNA polymerase

Proc Natl Acad Sci U S A. 1985 Apr;82(8):2354-8. doi: 10.1073/pnas.82.8.2354.


The bulk of an open reading frame extending from map coordinates 23.3 to 14.2 in region E2b of the adenoviral genome has been cloned and expressed from a chimeric plasmid in Escherichia coli. The cloning strategy used created a fusion protein of 124,000 daltons, which contained greater than 98% adenovirus-encoded sequences. Antiserum raised against this protein reacted with the authentic 140,000-dalton adenovirus DNA polymerase. Another serum raised against a synthetic hexapeptide whose sequence corresponded to the predicted carboxyl terminus of adenovirus-encoded DNA polymerase also reacted with the fusion protein and authentic adenovirus DNA polymerase. These results demonstrate that the cloned region of DNA encodes the adenovirus DNA polymerase.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / immunology
  • Cloning, Molecular
  • DNA-Directed DNA Polymerase / genetics*
  • DNA-Directed DNA Polymerase / immunology
  • Escherichia coli / genetics*
  • Genes, Viral
  • Immunochemistry
  • Plasmids
  • Viral Proteins / genetics


  • Viral Proteins
  • DNA-Directed DNA Polymerase