Secreted phospholipases of the dimorphic fungus, Candida albicans; separation of three enzymes and some biological properties

Sabouraudia. 1985 Feb;23(1):47-54. doi: 10.1080/00362178585380081.

Abstract

Several phospholipases are secreted into the culture medium by growing yeast cells of Candida albicans 3125. DEAE-Sephadex column chromatography of concentrated culture filtrate revealed three separable fractions with phospholipase activities. Analysis of products of hydrolysis showed that the enzyme activities were lysophospholipase, lysophospholipase-transacylase and a phospholipase B.

MeSH terms

  • Acyltransferases / metabolism*
  • Candida albicans / enzymology*
  • Candida albicans / growth & development
  • Candida albicans / metabolism
  • Chromatography, Ion Exchange
  • Fungal Proteins / metabolism*
  • Hydrogen-Ion Concentration
  • Lysophospholipase / metabolism*
  • Multienzyme Complexes / metabolism*
  • Phosphatidylcholines / metabolism
  • Phospholipases / metabolism*

Substances

  • Fungal Proteins
  • Multienzyme Complexes
  • Phosphatidylcholines
  • Acyltransferases
  • Phospholipases
  • Lysophospholipase
  • lysophospholipase-transacylase