Alpha-linolenic acid selectively inhibits the contraction of pig coronary arteries mediated through prostanoid TP receptors

J Pharmacol Sci. 2024 Aug;155(4):148-151. doi: 10.1016/j.jphs.2024.06.001. Epub 2024 Jun 4.

Abstract

We examined the inhibitory effects of α-linolenic acid (ALA) on the contractions of pig coronary arteries. ALA concentration-dependently inhibited the contractions elicited by U46619 and prostaglandin F2α without affecting those elicited by 80 mM KCl, histamine, acetylcholine, and serotonin. ALA rightward shifted the concentration-response curve of U46619, and Schild plot analysis revealed that ALA competitively antagonized U46619. Furthermore, ALA inhibited the increase in intracellular Ca2+ concentration caused by TP receptor stimulation but not that caused by FP receptor stimulation. These results suggest that ALA behaves as a selective antagonist of TP receptors in coronary arteries.

Keywords: Pig coronary artery; Prostanoid TP receptor; α-Linolenic acid.

MeSH terms

  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid* / pharmacology
  • Animals
  • Calcium* / metabolism
  • Coronary Vessels* / drug effects
  • Dinoprost / pharmacology
  • Dose-Response Relationship, Drug
  • Male
  • Muscle Contraction / drug effects
  • Receptors, Thromboxane* / antagonists & inhibitors
  • Receptors, Thromboxane* / metabolism
  • Swine
  • alpha-Linolenic Acid* / pharmacology

Substances

  • alpha-Linolenic Acid
  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
  • Calcium
  • Receptors, Thromboxane
  • Dinoprost