Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis

Cell. 1985 May;41(1):177-90. doi: 10.1016/0092-8674(85)90072-8.

Abstract

The cell type-specific expression of the major nuclear lamina polypeptides ("lamins") during development of Xenopus was studied using two monoclonal antibodies (L(0)46F7: specific for LIII, the single lamin of oocytes; PKB8: specific for LI and LII of some somatic cells). In the oocyte, LIII localizes in the nuclear polymer, but upon nuclear envelope breakdown it is solubilized to a form sedimenting at 9 S. In early embryos, LIII contributes to nuclear lamina formation until its depletion. Correspondingly, LI and LII begin to be expressed at a specific point in embryogenesis and appear to be integrated with LIII into a common lamina structure. Later in development, LIII reappears as a prominent nuclear lamina protein but only in certain cells (neurons, muscle cells, and diplotene oocytes). We conclude that amphibian lamins represent a family of proteins expressed in relation to certain programs of cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Embryo, Nonmammalian / metabolism
  • Female
  • Fluorescent Antibody Technique
  • Lamins
  • Meiosis
  • Metamorphosis, Biological
  • Muscles / metabolism
  • Neurons / metabolism
  • Nucleoproteins / analysis
  • Nucleoproteins / biosynthesis*
  • Oocytes / metabolism*
  • Oogenesis
  • Ovary / growth & development
  • Ovary / metabolism
  • Xenopus laevis / embryology
  • Xenopus laevis / growth & development*
  • Xenopus laevis / metabolism

Substances

  • Lamins
  • Nucleoproteins