Establishment of Cardiac Laterality

Abstract

Formation of the vertebrate heart with its complex arterial and venous connections is critically dependent on patterning of the left-right axis during early embryonic development. Abnormalities in left-right patterning can lead to a variety of complex life-threatening congenital heart defects. A highly conserved pathway responsible for left-right axis specification has been uncovered. This pathway involves initial asymmetric activation of a nodal signaling cascade at the embryonic node, followed by its propagation to the left lateral plate mesoderm and activation of left-sided expression of the Pitx2 transcription factor specifying visceral organ asymmetry. Intriguingly, recent work suggests that cardiac laterality is encoded by intrinsic cell and tissue chirality independent of Nodal signaling. Thus, Nodal signaling may be superimposed on this intrinsic chirality, providing additional instructive cues to pattern cardiac situs. The impact of intrinsic chirality and the perturbation of left-right patterning on myofiber organization and cardiac function warrants further investigation. We summarize recent insights gained from studies in animal models and also some human clinical studies in a brief overview of the complex processes regulating cardiac asymmetry and their impact on cardiac function and the pathogenesis of congenital heart defects.

Keywords: Atrial isomerism; Atrial situs; Bone morphogenetic protein; Cardiac asymmetry; Dextrocardia; Great arteries; Heart looping; Heterotaxy; Joubert syndrome; Kartagener syndrome; Laterality defects; Left lateral plate mesoderm; Left–right patterning; Mesocardia; Motile cilia; Pitx2; Primary cilia; Primary ciliary dyskinesia; Situs inversus; Situs solitus; Zebrafish; Zic family members.