Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities

Immunology. 1985 May;55(1):23-30.


In the present study, the non-lymphoid cell of the marginal zone is described: the marginal zone macrophage, identified by a monoclonal antibody, ERTR9. The characteristics of these cells were investigated by use of a two-step immunoperoxidase method (on light and ultrastructural level) in combination with enzyme-cytochemical techniques and functional tests. The marginal zone macrophage appears to be a large cell with close contacts between its cell processes and surrounding marginal zone B cells, and with a well-developed phagocytic capacity. These ERTR9-positive macrophages are capable of selective uptake and retention of i.v. injected FITC-Ficoll and are negative for Ia antigen as well as for the macrophage-related F4/80 antigen. By these characteristics, they constitute a subpopulation of non-lymphoid cells of spleen unique for the marginal zone.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Ficoll / analogs & derivatives
  • Ficoll / metabolism
  • Fluorescein-5-isothiocyanate* / analogs & derivatives*
  • Fluoresceins / metabolism
  • Immunoenzyme Techniques
  • Lymphoid Tissue / immunology
  • Macrophages / enzymology
  • Macrophages / immunology*
  • Macrophages / ultrastructure
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron
  • Phagocytosis
  • Spleen / immunology*


  • Antibodies, Monoclonal
  • Fluoresceins
  • fluorescein 5-isothiocyanate-Ficoll
  • Ficoll
  • Fluorescein-5-isothiocyanate