Characterization of Clostridia Isolated From Faeces of Limited Flora Mice and Their Effect on Caecal Size When Associated With Germ-Free Mice

Lab Anim. 1985 Apr;19(2):111-8. doi: 10.1258/002367785780942589.


115 strains of clostridia were accumulated from 3 separate isolations from the faeces of 1 limited flora (LF) mouse produced by inoculation of germ-free mice with chloroform-treated faeces of conventional mice. These strains were divided into 36 types on the basis of conventional biochemical characteristics. There was some variation in types isolated on the 3 occasions. Although 3 groups of polyassociated (PA) mice were produced from mixing 46 monoassociated mice, each inoculated with 1 of 46 strains of 36 bacterial types, the caecal size of PA mice was still greater than that of the control mice prepared by inoculation of chloroform-treated faeces of an LF mouse. After PA mice of each group were housed together, the caecal size became smaller and was only slightly larger than the control.

MeSH terms

  • Acetates / metabolism
  • Animals
  • Bacteriological Techniques
  • Butyrates / metabolism
  • Cecum / anatomy & histology*
  • Clostridium / classification*
  • Clostridium / isolation & purification
  • Clostridium / metabolism
  • Feces / microbiology*
  • Formates / metabolism
  • Germ-Free Life*
  • Male
  • Mice


  • Acetates
  • Butyrates
  • Formates