Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

D N Kisakov; L A Kisakova; S V Sharabrin; V A Yakovlev; E V Tigeeva; M B Borgoyakova; E V Starostina; A V Zaikovskaya; A P Rudometov; N B Rudometova; L I Karpenko; A A Ilyichev

doi:10.1007/s10517-024-06107-x

Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

Bull Exp Biol Med. 2024 Apr;176(6):776-780. doi: 10.1007/s10517-024-06107-x. Epub 2024 Jun 19.

Authors

Affiliations

¹ State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. def_2003@mail.ru.
² State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.

PMID: 38896316
DOI: 10.1007/s10517-024-06107-x

Abstract

We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.

Keywords: delivery methods; jet injection; mRNA vaccines; receptor-binding domain of the S protein of the SARS-CoV-2 virus.

MeSH terms

Animals
Antibodies, Neutralizing* / immunology
Antibodies, Viral* / immunology
COVID-19 Vaccines* / administration & dosage
COVID-19 Vaccines* / immunology
COVID-19* / immunology
COVID-19* / prevention & control
COVID-19* / virology
Female
Humans
Injections, Intramuscular
Injections, Jet
Mice
Mice, Inbred BALB C*
RNA, Messenger / genetics
RNA, Messenger / immunology
SARS-CoV-2* / genetics
SARS-CoV-2* / immunology
Spike Glycoprotein, Coronavirus* / genetics
Spike Glycoprotein, Coronavirus* / immunology
T-Lymphocytes / immunology
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / immunology
mRNA Vaccines

Substances

Spike Glycoprotein, Coronavirus
Antibodies, Viral
COVID-19 Vaccines
Antibodies, Neutralizing
spike protein, SARS-CoV-2
mRNA Vaccines
RNA, Messenger
Vaccines, Synthetic