Isolation, Characterization, and Utilization of Human Skin Basal and Suprabasal Epidermal Stem Cells

Methods Mol Biol. 2024 Jun 22. doi: 10.1007/7651_2024_551. Online ahead of print.

Abstract

Studying human skin biology can aid in comprehending the pathophysiology of skin diseases and developing novel cell-based therapies, including tissue engineering approaches. This chapter provides a comprehensive guide of methods to determine human skin samples from the perspective of their cellular compositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.

Keywords: Homeostasis; Integrin beta 4; Interfollicular epidermis; Keratinocyte; Regeneration; Stem cells.