A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat

Biochem J. 1985 May 15;228(1):103-10. doi: 10.1042/bj2280103.

Abstract

A quantitative method allowing determination of glucose metabolism in vivo in muscles and white adipose tissue of the anaesthetized rat is presented. A tracer dose of 2-deoxy[3H]glucose was injected intravenously in an anaesthetized rat and the concentration of 2-deoxy[3H]glucose was monitored in arterial blood. After 30-80 min, three muscles, the soleus, the extensor digitorum longus and the epitrochlearis, periovarian white adipose tissue and brain were sampled and analysed for their content of 2-deoxy[3H]glucose 6-phosphate. This content could be related to glucose utilization during the same time period, since (1) the integral of the decrease of 2-deoxy[3H]glucose in arterial blood was known and (2) correction factors for the analogue effect of 2-deoxyglucose compared with glucose in the transport and phosphorylation steps were determined from experiments in vitro. Glucose utilization was then measured by this technique in the tissues of post-absorptive rats in the basal state (0.1 munit of insulin/ml of plasma) or during euglycaemic-hyperinsulinaemic glucose clamp (8 munits of insulin/ml of plasma) and of 48 h-starved rats. Results corresponded qualitatively and quantitatively to the known physiological characteristics of the tissues studied.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Animals
  • Blood Glucose / metabolism
  • Deoxyglucose / metabolism
  • Female
  • Glucose / metabolism*
  • Hyperinsulinism / metabolism
  • Insulin / blood
  • Muscles / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Starvation / metabolism

Substances

  • Blood Glucose
  • Insulin
  • Deoxyglucose
  • Glucose